Tsuchikama K, Gooyit M, Harris TL, Zhu J, Globisch D, Kaufmann GF, Janda KD
Angew. Chem. Int. Ed. Engl. 55 (12) 4002-4006 [2016-03-14; online 2016-02-17]
Reported herein is that (4S)-4,5-dihydroxy-2,3-pentanedione (DPD) can undergo a previously undocumented non-enzymatic glycation reaction. Incubation of DPD with viral DNA or the antibiotic gramicidin S resulted in significant biochemical alterations. A protein-labeling method was consequently developed that facilitated the identification of unrecognized glycation targets of DPD in a prokaryotic system. These results open new avenues toward tracking and understanding the fate and function of the elusive quorum-sensing signaling molecule.