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Frawley AT, Wycisk V, Xiong Y, Galiani S, Sezgin E, Urbančič I, Vargas Jentzsch A, Leslie KG, Eggeling C, Anderson HL
Chem Sci 11 (33) 8955-8960 [2020-08-10; online 2020-08-10]
Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm).
PubMed 34123149
DOI 10.1039/d0sc02447c
Crossref 10.1039/d0sc02447c
pmc: PMC8163400
pii: d0sc02447c