Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad.

Frawley AT, Wycisk V, Xiong Y, Galiani S, Sezgin E, Urbančič I, Vargas Jentzsch A, Leslie KG, Eggeling C, Anderson HL

Chem Sci 11 (33) 8955-8960 [2020-08-10; online 2020-08-10]

Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm).

Erdinc Sezgin

SciLifeLab Fellow

PubMed 34123149

DOI 10.1039/d0sc02447c

Crossref 10.1039/d0sc02447c

pmc: PMC8163400
pii: d0sc02447c

Publications 9.5.0