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Münzel M, Globisch D, Trindler C, Carell T
Org. Lett. 12 (24) 5671-5673 [2010-12-17; online 2010-12-17]
5-Hydroxymethylcytosine ((5-HOMe)dC) was recently discovered as the sixth base in the mammalian genome. The development of a new phosphoramidite building block is reported, which allows efficient synthesis of (5-HOMe)dC containing DNA. Key steps of the synthesis are a palladium-catalyzed formylation and the simultaneous protection of a hydroxyl and amino group as a cyclic carbamate. DNA synthesis is possible under standard conditions, and deprotection can be carried out with dilute NaOH.
PubMed 21082782
DOI 10.1021/ol102408t
Crossref 10.1021/ol102408t