Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy.

Sezgin E, Schneider F, Zilles V, Urbančič I, Garcia E, Waithe D, Klymchenko AS, Eggeling C

Biophys. J. 113 (6) 1321-1330 [2017-09-19; online 2017-07-19]

The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids are packed. Polarity-sensitive dyes are powerful tools to characterize such lipid membrane order, employing, for example, confocal and two-photon microscopy. The investigation of potential nanodomains, however, requires the use of superresolution microscopy. Here, we test the performance of the polarity-sensitive membrane dyes Di-4-ANEPPDHQ, Di-4-AN(F)EPPTEA, and NR12S in superresolution stimulated emission depletion microscopy. Measurements on cell-derived membrane vesicles, in the plasma membrane of live cells, and on single virus particles, show the high potential of these dyes for probing nanoscale membrane heterogeneity.

Erdinc Sezgin

SciLifeLab Fellow

PubMed 28734477

DOI 10.1016/j.bpj.2017.06.050

Crossref 10.1016/j.bpj.2017.06.050

pii: S0006-3495(17)30700-2
pmc: PMC5607142

Publications 9.5.0