High performance micro-flow cytometer based on optical fibres.

Etcheverry S, Faridi A, Ramachandraiah H, Kumar T, Margulis W, Laurell F, Russom A

Sci Rep 7 (1) 5628 [2017-07-17; online 2017-07-17]

Flow cytometry is currently the gold standard for analysis of cells in the medical laboratory and biomedical research. Fuelled by the need of point-of-care diagnosis, a significant effort has been made to miniaturize and reduce cost of flow cytometers. However, despite recent advances, current microsystems remain less versatile and much slower than their large-scale counterparts. In this work, an all-silica fibre microflow cytometer is presented that measures fluorescence and scattering from particles and cells. It integrates cell transport in circular capillaries and light delivery by optical fibres. Single-stream cell focusing is performed by Elasto-inertial microfluidics to guarantee accurate and sensitive detection. The capability of this technique is extended to high flow rates (up to 800 µl/min), enabling a throughput of 2500 particles/s. The robust, portable and low-cost system described here could be the basis for a point-of-care flow cytometer with a performance comparable to commercial systems.

Affiliated researcher

PubMed 28717236

DOI 10.1038/s41598-017-05843-7

Crossref 10.1038/s41598-017-05843-7

pii: 10.1038/s41598-017-05843-7
pmc: PMC5514097

Publications 7.1.2