Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes.

Pereno V, Carugo D, Bau L, Sezgin E, Bernardino de la Serna J, Eggeling C, Stride E

ACS Omega 2 (3) 994-1002 [2017-03-31; online 2017-03-16]

Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles. Currently, however, it is expensive and time-consuming compared with other methods. In this study, we demonstrate that 1,2-dioleoyl- sn-glycero-3-phosphatidylcholine GUVs can be prepared readily at a fraction of the cost on stainless steel electrodes, such as commercially available syringe needles, without any evidence of lipid oxidation or hydrolysis.

Erdinc Sezgin

SciLifeLab Fellow

PubMed 28393132

DOI 10.1021/acsomega.6b00395

Crossref 10.1021/acsomega.6b00395

pmc: PMC5377273

Publications 9.5.0