Photoswitchable Spiropyran Dyads for Biological Imaging.

Xiong Y, Rivera-Fuentes P, Sezgin E, Vargas Jentzsch A, Eggeling C, Anderson HL

Org. Lett. 18 (15) 3666-3669 [2016-08-05; online 2016-07-26]

The synthesis of a small-molecule dyad consisting of a far-red-emitting silicon rhodamine dye that is covalently linked to a photochromic spironaphthothiopyran unit, which serves as a photoswitchable quencher, is reported. This system can be switched reversibly between the fluorescent and nonfluorescent states using visible light at wavelengths of 405 and 630 nm, respectively, and it works effectively in aqueous solution. Live-cell imaging demonstrates that this dyad has several desirable features, including excellent membrane permeability, fast and reversible modulation of fluorescence by visible light, and good contrast between the bright and dark states.

Erdinc Sezgin

PubMed 27456166

DOI 10.1021/acs.orglett.6b01717

Crossref 10.1021/acs.orglett.6b01717

pmc: PMC5010358