Inhibition of RPS6K reveals context-dependent Akt activity in luminal breast cancer cells.

Erdem C, Lee AV, Taylor DL, Lezon TR

PLoS Comput Biol 17 (6) e1009125 [2021-06-00; online 2021-06-30]

Aberrant signaling through insulin (Ins) and insulin-like growth factor I (IGF1) receptors contribute to the risk and advancement of many cancer types by activating cell survival cascades. Similarities between these pathways have thus far prevented the development of pharmacological interventions that specifically target either Ins or IGF1 signaling. To identify differences in early Ins and IGF1 signaling mechanisms, we developed a dual receptor (IGF1R & InsR) computational response model. The model suggested that ribosomal protein S6 kinase (RPS6K) plays a critical role in regulating MAPK and Akt activation levels in response to Ins and IGF1 stimulation. As predicted, perturbing RPS6K kinase activity led to an increased Akt activation with Ins stimulation compared to IGF1 stimulation. Being able to discern differential downstream signaling, we can explore improved anti-IGF1R cancer therapies by eliminating the emergence of compensation mechanisms without disrupting InsR signaling.

Cemal Erdem

DDLS Fellow

PubMed 34191793

DOI 10.1371/journal.pcbi.1009125

Crossref 10.1371/journal.pcbi.1009125

pmc: PMC8277016
pii: PCOMPBIOL-D-21-00080

Publications 9.5.0