Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.

Pennacchietti F, Serebrovskaya EO, Faro AR, Shemyakina II, Bozhanova NG, Kotlobay AA, Gurskaya NG, Bodén A, Dreier J, Chudakov DM, Lukyanov KA, Verkhusha VV, Mishin AS, Testa I

Nat. Methods 15 (8) 601-604 [2018-08-00; online 2018-07-09]

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.

Affiliated researcher

Ilaria Testa

SciLifeLab Fellow

PubMed 29988095

DOI 10.1038/s41592-018-0052-9

Crossref 10.1038/s41592-018-0052-9

pii: 10.1038/s41592-018-0052-9

Publications 9.5.0