In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta.

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Pernemalm M, Sandberg A, Zhu Y, Boekel J, Tamburro D, Schwenk JM, Björk A, Wahren-Herlenius M, Åmark H, Östenson CG, Westgren M, Lehtiö J

Elife 8 (-) - [2019-04-08; online 2019-04-08]

Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We further show that our method has the ability to detect both low abundance tissue-annotated proteins and phosphorylated proteins in plasma, as well as quantitate differences in plasma proteomes between the mother and the newborn as well as changes related to pregnancy.

PubMed 30958262

DOI 10.7554/eLife.41608

Crossref 10.7554/eLife.41608

pmc: PMC6519984
pii: 41608