Multiplexed ChIP-Seq Using Direct Nucleosome Barcoding: A Tool for High-Throughput Chromatin Analysis.

Chabbert CD, Adjalley SH, Steinmetz LM, Pelechano V

Methods in molecular biology (Clifton, N.J.) 1689 (-) 177-194 [2017-10-14; online 2017-10-14]

Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) or microarray hybridization (ChIP-on-chip) are standard methods for the study of transcription factor binding sites and histone chemical modifications. However, these approaches only allow profiling of a single factor or protein modification at a time.In this chapter, we present Bar-ChIP, a higher throughput version of ChIP-Seq that relies on the direct ligation of molecular barcodes to chromatin fragments. Bar-ChIP enables the concurrent profiling of multiple DNA-protein interactions and is therefore amenable to experimental scale-up, without the need for any robotic instrumentation.

SciLifeLab Fellow

Vicent Pelechano

PubMed 29027175

DOI 10.1007/978-1-4939-7380-4_16

Crossref 10.1007/978-1-4939-7380-4_16

Publications 9.5.0