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Archer A, Kutter C, Williams C
Methods in molecular biology (Clifton, N.J.) 2418 (-) 313-343 [2022-02-05; online 2022-02-05]
MicroRNAs play critical roles through their impact on posttranscriptional gene regulation. In cancer, they can act as oncogenes or tumor suppressors and can also function as biomarkers. Here, we describe a method for robust characterization of estrogen-regulated microRNA profiles. The activity of estrogen is mediated by two nuclear receptors, estrogen receptor alpha and estrogen receptor beta, and a transmembrane G-protein coupled estrogen receptor 1. This chapter details how to prepare cells for optimal estrogen response, directions for estrogen treatment, RNA extraction, different microRNA profiling approaches, and subsequent confirmations.
PubMed 35119673
DOI 10.1007/978-1-0716-1920-9_18
Crossref 10.1007/978-1-0716-1920-9_18