A versatile genome-scale PCR-based pipeline for high-definition DNA FISH

Bienko M, Crosetto N, Teytelman L, Klemm S, Itzkovitz S, van Oudenaarden A

Nat Methods 10 (2) 122-124 [2013-02-00; online 2012-12-23]

We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.

Magda Bienko

SciLifeLab Fellow

PubMed 23263692

DOI 10.1038/nmeth.2306

Crossref 10.1038/nmeth.2306

Publications 8.1.0