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Wagner AK, Kadri N, Tibbitt C, van de Ven K, Bagawath-Singh S, Oliinyk D, LeGresley E, Campbell N, Trittel S, Riese P, Ribacke U, Sandalova T, Achour A, Kärre K, Chambers BJ
iScience 25 (10) 105137 [2022-10-21; online 2022-09-16]
Although PD-1 was shown to be a hallmark of T cells exhaustion, controversial studies have been reported on the role of PD-1 on NK cells. Here, we found by flow cytometry and single cell RNA sequencing analysis that PD-1 can be expressed on MHC class I-deficient tumor-infiltrating NK cells in vivo. We also demonstrate distinct alterations in the phenotype of PD-1-deficient NK cells and a more mature phenotype which might reduce their capacity to migrate and kill in vivo. Tumor-infiltrating NK cells that express PD-1 were highly associated with the expression of CXCR6. Furthermore, our results demonstrate that PD-L1 molecules in membranes of PD-1-deficient NK cells migrate faster than in NK cells from wild-type mice, suggesting that PD-1 and PD-L1 form cis interactions with each other on NK cells. These data demonstrate that there may be a role for the PD-1/PD-L1 axis in tumor-infiltrating NK cells in vivo.
PubMed 36185379
DOI 10.1016/j.isci.2022.105137
Crossref 10.1016/j.isci.2022.105137
pmc: PMC9523278
pii: S2589-0042(22)01409-2