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Wissink EM, Vihervaara A, Tippens ND, Lis JT
Nat. Rev. Genet. 20 (12) 705-723 [2019-12-00; online 2019-08-09]
The programmes that direct an organism's development and maintenance are encoded in its genome. Decoding of this information begins with regulated transcription of genomic DNA into RNA. Although transcription and its control can be tracked indirectly by measuring stable RNAs, it is only by directly measuring nascent RNAs that the immediate regulatory changes in response to developmental, environmental, disease and metabolic signals are revealed. Multiple complementary methods have been developed to quantitatively track nascent transcription genome-wide at nucleotide resolution, all of which have contributed novel insights into the mechanisms of gene regulation and transcription-coupled RNA processing. Here we critically evaluate the array of strategies used for investigating nascent transcription and discuss the recent conceptual advances they have provided.
PubMed 31399713
DOI 10.1038/s41576-019-0159-6
Crossref 10.1038/s41576-019-0159-6
mid: NIHMS1048955
pmc: PMC6858503
pii: 10.1038/s41576-019-0159-6