Application of high-throughput 5'P sequencing for the study of co-translational mRNA decay.

Zhang Y, Pelechano V

STAR Protoc 2 (2) 100447 [2021-06-18; online 2021-03-31]

mRNA degradation is connected to the translation process up to the degree that 5'-3' mRNA degradation follows the last translating ribosome. To study 5'-3'co-translational mRNA decay and the associated ribosome dynamics, here we present an improved high-throughput 5'P degradome RNA sequencing protocol (HT-5Pseq). We exemplify its application in Saccharomyces cerevisiae, but in principle, it could be applied to any other eukaryotic organism. HT-5Pseq is easy, scalable, and uses affordable duplex-specific nuclease-based rRNA depletion. For complete details on the use and execution of this protocol, please refer to Zhang and Pelechano (2021).

SciLifeLab Fellow

Vicent Pelechano

PubMed 33870233

DOI 10.1016/j.xpro.2021.100447

Crossref 10.1016/j.xpro.2021.100447

pii: S2666-1667(21)00154-4
pmc: PMC8044721

Publications 9.5.0