Adaptive lipid packing and bioactivity in membrane domains.

Sezgin E, Gutmann T, Buhl T, Dirkx R, Grzybek M, Coskun Ü, Solimena M, Simons K, Levental I, Schwille P

PLoS ONE 10 (4) e0123930 [2015-04-23; online 2015-04-23]

Lateral compositional and physicochemical heterogeneity is a ubiquitous feature of cellular membranes on various length scales, from molecular assemblies to micrometric domains. Segregated lipid domains of increased local order, referred to as rafts, are believed to be prominent features in eukaryotic plasma membranes; however, their exact nature (i.e. size, lifetime, composition, homogeneity) in live cells remains difficult to define. Here we present evidence that both synthetic and natural plasma membranes assume a wide range of lipid packing states with varying levels of molecular order. These states may be adapted and specifically tuned by cells during active cellular processes, as we show for stimulated insulin secretion. Most importantly, these states regulate both the partitioning of molecules between coexisting domains and the bioactivity of their constituent molecules, which we demonstrate for the ligand binding activity of the glycosphingolipid receptor GM1. These results confirm the complexity and flexibility of lipid-mediated membrane organization and reveal mechanisms by which this flexibility could be functionalized by cells.

Erdinc Sezgin

SciLifeLab Fellow

PubMed 25905447

DOI 10.1371/journal.pone.0123930

Crossref 10.1371/journal.pone.0123930

pii: PONE-D-14-53017
pmc: PMC4408024

Publications 9.5.0