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Mayor-Ruiz C, Dominguez O, Fernandez-Capetillo O
J. Mol. Biol. 429 (18) 2780-2789 [2017-09-01; online 2017-08-04]
The development of haploid mammalian cell lines, coupled to next-generation sequencing, has recently facilitated forward genetic screenings in mammals. For mutagenesis, retrovirus- or transposon-based gene traps are frequently used. Current methods to map gene-trap insertions are based on inverse or splinkerette PCR, which despite their efficacy are prone to artifacts and do not provide information on expression of the targeted gene. Here, we describe a new RNA sequencing-based method (Trap
PubMed 28782559
DOI 10.1016/j.jmb.2017.07.020
Crossref 10.1016/j.jmb.2017.07.020
pii: S0022-2836(17)30370-4
pmc: PMC5695663
mid: EMS74344