Detection of rotavirus using padlock probes and rolling circle amplification.

Mezger A, Öhrmalm C, Herthnek D, Blomberg J, Nilsson M

PLoS ONE 9 (11) e111874 [2014-11-04; online 2014-11-04]

Rotavirus infections are one of the most common reasons for hospitalizations due to gastrointestinal diseases. Rotavirus is often diagnosed by latex agglutination assay, chromatography immunoassay, or by electron microscopy, which are all quite insensitive. Reverse transcription polymerase chain reaction, on the other hand, is very sensitive to variations at the genomic level. We developed a novel assay based on a set of 58 different padlock probes with a detection limit of 1,000 copies. Twenty-two patient samples were analyzed and the assay showed high concordance with a PCR-based assay. In summary, we present a new assay for sensitive and variation tolerant detection of rotavirus.

Affiliated researcher

PubMed 25369034

DOI 10.1371/journal.pone.0111874

Crossref 10.1371/journal.pone.0111874

pii: PONE-D-14-15215
pmc: PMC4219791