The histone H2A isoform Hist2h2ac is a novel regulator of proliferation and epithelial-mesenchymal transition in mammary epithelial and in breast cancer cells.

Monteiro FL, Vitorino R, Wang J, Cardoso H, Laranjeira H, Simões J, Caldas M, Henrique R, Amado F, Williams C, Jerónimo C, Helguero LA

Cancer Lett. 396 (-) 42-52 [2017-06-28; online 2017-03-11]

Proliferation and differentiation are controlled through chromatin remodelling. Therefore, there is an enormous biological significance and clinical value in understanding how specific signalling pathways are affected by histone replacement in the nucleosome. In this work, mass spectrometry was used to screen HC11 mammary epithelial cells for changes in histone levels throughout cell differentiation. The canonical histone isoform Histone H2A type 2-C (Hist2h2ac) was found only in undifferentiated/proliferating cells. Hist2h2ac mRNA was induced by EGF, specifically in the CD24+/CD29hi/DC44hi cell subpopulation. Hist2h2ac mRNA was increased by MEK

Affiliated researcher

PubMed 28288875

DOI 10.1016/j.canlet.2017.03.007

Crossref 10.1016/j.canlet.2017.03.007

pii: S0304-3835(17)30171-4

Publications 9.5.0