An image analysis toolbox for high-throughput C. elegans assays.

Wählby C, Kamentsky L, Liu ZH, Riklin-Raviv T, Conery AL, O'Rourke EJ, Sokolnicki KL, Visvikis O, Ljosa V, Irazoqui JE, Golland P, Ruvkun G, Ausubel FM, Carpenter AE

Nat. Methods 9 (7) 714-716 [2012-04-22; online 2012-04-22]

We present a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and imaging systems. This WormToolbox is available through the open-source CellProfiler project and enables objective scoring of whole-worm high-throughput image-based assays of C. elegans for the study of diverse biological pathways that are relevant to human disease.

Affiliated researcher

PubMed 22522656

DOI 10.1038/nmeth.1984

Crossref 10.1038/nmeth.1984

pii: nmeth.1984
pmc: PMC3433711
mid: NIHMS397906

Publications 9.5.0