Rapid screening of complex DNA samples by single-molecule amplification and sequencing.

Huang J, Zheng Z, Andersson AF, Engstrand L, Ye W

PLoS ONE 6 (5) e19723 [2011-05-19; online 2011-05-19]

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.

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PubMed 21625543

DOI 10.1371/journal.pone.0019723

Crossref 10.1371/journal.pone.0019723

PONE-D-11-05086

pmc PMC3098247