GradientOptimizer: an open-source graphical environment for calculating optimized gradients in reversed-phase liquid chromatography.

Moruz L, Käll L

Proteomics 14 (12) 1464-1466 [2014-06-00; online 2014-05-15]

We here present GradientOptimizer, an intuitive, lightweight graphical user interface to design nonlinear gradients for separation of peptides by reversed-phase liquid chromatography. The software allows to calculate three types of nonlinear gradients, each of them optimizing a certain retention time distribution of interest. GradientOptimizer is straightforward to use, requires minimum processing of the input files, and is supported under Windows, Linux, and OS X platforms. The software is open-source and can be downloaded under an Apache 2.0 license at

Affiliated researcher

PubMed 24700534

DOI 10.1002/pmic.201400036

Crossref 10.1002/pmic.201400036

Publications 9.5.0