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Zelenin S, Hansson J, Ardabili S, Ramachandraiah H, Brismar H, Russom A
Biotechnol. Lett. 37 (4) 825-830 [2015-04-00; online 2014-11-21]
Blood-stream infections (BSI) remain a major health challenge, with an increasing incidence worldwide and a high mortality rate. Early treatment with appropriate antibiotics can reduce BSI-related morbidity and mortality, but success requires rapid identification of the infecting organisms. The rapid, culture-independent diagnosis of BSI could be significantly facilitated by straightforward isolation of highly purified bacteria from whole blood. We present a microfluidic-based, sample-preparation system that rapidly and selectively lyses all blood cells while it extracts intact bacteria for downstream analysis. Whole blood is exposed to a mild detergent, which lyses most blood cells, and then to osmotic shock using deionized water, which eliminates the remaining white blood cells. The recovered bacteria are 100% viable, which opens up possibilities for performing drug susceptibility tests and for nucleic-acid-based molecular identification.
PubMed 25413883
DOI 10.1007/s10529-014-1734-8
Crossref 10.1007/s10529-014-1734-8