Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes.

Balzarotti F, Eilers Y, Gwosch KC, GynnÄ AH, Westphal V, Stefani FD, Elf J, Hell SW

Science 355 (6325) 606-612 [2017-02-10; online 2016-12-22]

We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. In our experiments, 22 times fewer fluorescence photons are required as compared to popular centroid localization. In superresolution microscopy, MINFLUX attained ~1-nm precision, resolving molecules only 6 nanometers apart. MINFLUX tracking of single fluorescent proteins increased the temporal resolution and the number of localizations per trace by a factor of 100, as demonstrated with diffusing 30

Affiliated researcher

PubMed 28008086

DOI 10.1126/science.aak9913

Crossref 10.1126/science.aak9913

pii: science.aak9913

Publications 9.5.0