Rapid identification of bio-molecules applied for detection of biosecurity agents using rolling circle amplification.

Göransson J, Ke R, Nong RY, Howell WM, Karman A, Grawé J, Stenberg J, Granberg M, Elgh M, Herthnek D, Wikström P, Jarvius J, Nilsson M

PLoS ONE 7 (2) e31068 [2012-02-22; online 2012-02-22]

Detection and identification of pathogens in environmental samples for biosecurity applications are challenging due to the strict requirements on specificity, sensitivity and time. We have developed a concept for quick, specific and sensitive pathogen identification in environmental samples. Target identification is realized by padlock- and proximity probing, and reacted probes are amplified by RCA (rolling-circle amplification). The individual RCA products are labeled by fluorescence and enumerated by an instrument, developed for sensitive and rapid digital analysis. The concept is demonstrated by identification of simili biowarfare agents for bacteria (Escherichia coli and Pantoea agglomerans) and spores (Bacillus atrophaeus) released in field.

Affiliated researcher

PubMed 22383994

DOI 10.1371/journal.pone.0031068

Crossref 10.1371/journal.pone.0031068

pii: PONE-D-11-09305
pmc: PMC3285169

Publications 9.5.0