Photocontrol of cell adhesion processes: model studies with cyclic azobenzene-RGD peptides.

Schütt M, Krupka SS, Milbradt AG, Deindl S, Sinner EK, Oesterhelt D, Renner C, Moroder L

Chemistry & biology 10 (6) 487-490 [2003-06-00; online 2003-07-03]

A photoresponsive integrin ligand was synthesized by backbone-cyclization of a heptapeptide containing the integrin binding motif Arg-Gly-Asp (RGD) with 4-(aminomethyl)phenylazobenzoic acid (AMPB). Surface plasmon enhanced fluorescence spectroscopy showed that binding of the azobenzene peptide to alpha(v)beta(3) integrin depends on the photoisomeric state of the peptide chromophore. The higher affinity of the trans isomer could be rationalized by comparing the NMR conformations of the cis and trans isomers with the recently solved X-ray structure of a cyclic RGD-pentapeptide bound to integrin.

SciLifeLab Fellow

Sebastian Deindl

PubMed 12837381

DOI 10.1016/s1074-5521(03)00128-5

Crossref 10.1016/s1074-5521(03)00128-5

pii: S1074552103001285

Publications 9.5.0