JSON
Nat. Methods 18 (4) 358-365 [2021-04-00; online 2021-02-15]
Mapping a genetic perturbation to a change in phenotype is at the core of biological research. Advances in microscopy have transformed these studies, but they have largely been confined to examining a few strains or cell lines at a time. In parallel, there has been a revolution in creating synthetic libraries of genetically altered cells with relative ease. Here we describe methods that combine these powerful tools to perform live-cell imaging of pool-generated strain libraries for improved biological discovery.
PubMed 33589838
DOI 10.1038/s41592-020-01053-8
Crossref 10.1038/s41592-020-01053-8
pii: 10.1038/s41592-020-01053-8