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Li X, Visser S, Son JH, Geuverink E, Kıvanç EN, Wu Y, Schmeing S, Pippel M, Anvar SY, Schenkel MA, Marec F, Robinson MD, Meisel RP, Wimmer EA, van de Zande L, Bopp D, Beukeboom LW
Nat Commun 15 (1) 5984 [2024-07-16; online 2024-07-16]
Houseflies provide a good experimental model to study the initial evolutionary stages of a primary sex-determining locus because they possess different recently evolved proto-Y chromosomes that contain male-determining loci (M) with the same male-determining gene, Mdmd. We investigate M-loci genomically and cytogenetically revealing distinct molecular architectures among M-loci. M on chromosome V (MV) has two intact Mdmd copies in a palindrome. M on chromosome III (MIII) has tandem duplications containing 88 Mdmd copies (only one intact) and various repeats, including repeats that are XY-prevalent. M on chromosome II (MII) and the Y (MY) share MIII-like architecture, but with fewer repeats. MY additionally shares MV-specific sequence arrangements. Based on these data and karyograms using two probes, one derives from MIII and one Mdmd-specific, we infer evolutionary histories of polymorphic M-loci, which have arisen from unique translocations of Mdmd, embedded in larger DNA fragments, and diverged independently into regions of varying complexity.
PubMed 39013946
DOI 10.1038/s41467-024-50390-1
Crossref 10.1038/s41467-024-50390-1
pmc: PMC11252125
pii: 10.1038/s41467-024-50390-1
BioProject: PRJNA1013067
BioProject: PRJNA1072234
GENBANK: JAVQME000000000
GENBANK: JAZGUT000000000
GENBANK: JAVVNY000000000