The application of microbeads to microfluidic systems for enhanced detection and purification of biomolecules.

Pinto IF, Caneira CR, Soares RR, Madaboosi N, Aires-Barros MR, Conde JP, Azevedo AM, Chu V

Methods 116 (-) 112-124 [2017-03-01; online 2016-12-10]

This paper describes microbead-based microfluidic systems. Several aspects of bead assays in microfluidics make them advantageous for bioassays in simple microchannels, including enhanced surface-to-volume ratio, improved molecular recognition reaction efficiency, and the wide range of surface functionalization available with commercial microbeads. Two-level SU-8 molds are used to fabricate PDMS microchannels that can hydrodynamically trap different types of microbeads, with characteristic dimensions of tens of microns. The use of these microbead-based microfluidic systems in the biosensing of antibodies, toxins and nucleic acids, as well as in antibody purification will be presented and discussed in this paper.

Affiliated researcher

PubMed 27965121

DOI 10.1016/j.ymeth.2016.12.005

Crossref 10.1016/j.ymeth.2016.12.005

pii: S1046-2023(16)30476-5

Publications 7.1.2