A short C-terminal tail prevents mis-targeting of hydrophobic mitochondrial membrane proteins to the ER.
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Reithinger JH, Yim C, Park K, Björkholm P, von Heijne G, Kim H
FEBS Lett. 587 (21) 3480-3486 [2013-11-01; online 2013-09-18]
Sdh3/Shh3, a subunit of mitochondrial succinate dehydrogenase, contains transmembrane domains with a hydrophobicity comparable to that of endoplasmic reticulum (ER) proteins. Here, we show that a C-terminal reporter fusion to Sdh3/Shh3 results in partial mis-targeting of the protein to the ER. This mis-targeting is mediated by the signal recognition particle (SRP) and depends on the length of the C-terminal tail. These results imply that if nuclear-encoded mitochondrial proteins contain strongly hydrophobic transmembrane domains and a long C-terminal tail, they have the potential to be recognized by SRP and mis-targeted to the ER.
PubMed 24055247
DOI 10.1016/j.febslet.2013.08.041
Crossref 10.1016/j.febslet.2013.08.041
pii: S0014-5793(13)00670-4