rsEGFP2 enables fast RESOLFT nanoscopy of living cells.

Grotjohann T, Testa I, Reuss M, Brakemann T, Eggeling C, Hell SW, Jakobs S

Elife 1 (-) e00248 [2012-12-31; online 2012-12-31]

The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25-250 times faster recordings.DOI:

Affiliated researcher

PubMed 23330067

DOI 10.7554/eLife.00248

Crossref 10.7554/eLife.00248

pii: 00248
pmc: PMC3534202

Publications 9.5.0