Probing physical properties of a DNA-protein complex using nanofluidic channels.

Frykholm K, Alizadehheidari M, Fritzsche J, Wigenius J, Modesti M, Persson F, Westerlund F

Small 10 (5) 884-887 [2014-03-12; online 2014-01-02]

A method to investigate physical properties of a DNA-protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double-stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces.

Affiliated researcher

PubMed 24382826

DOI 10.1002/smll.201302028

Crossref 10.1002/smll.201302028