Expanding the product portfolio of fungal type I fatty acid synthases.

Zhu Z, Zhou YJ, Krivoruchko A, Grininger M, Zhao ZK, Nielsen J

Nat. Chem. Biol. 13 (4) 360-362 [2017-04-00; online 2017-02-20]

Fungal type I fatty acid synthases (FASs) are mega-enzymes with two separated, identical compartments, in which the acyl carrier protein (ACP) domains shuttle substrates to catalytically active sites embedded in the chamber wall. We devised synthetic FASs by integrating heterologous enzymes into the reaction chambers and demonstrated their capability to convert acyl-ACP or acyl-CoA from canonical fatty acid biosynthesis to short/medium-chain fatty acids and methyl ketones.

Affiliated researcher

PubMed 28218911

DOI 10.1038/nchembio.2301

Crossref 10.1038/nchembio.2301

pii: nchembio.2301