Bactobolin A Binds to a Site on the 70S Ribosome Distinct from Previously Seen Antibiotics

Amunts A, Fiedorczuk K, Truong TT, Chandler J, Greenberg EP, Ramakrishnan V

Journal of Molecular Biology 427 (4) 753-755 [2015-02-00; online 2015-02-00]

The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-Å-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations.

Alexey Amunts

SciLifeLab Fellow

PubMed 25562208

DOI 10.1016/j.jmb.2014.12.018

Crossref 10.1016/j.jmb.2014.12.018

Publications 9.5.0