A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitidis.
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Rivas L, Reuterswärd P, Rasti R, Herrmann B, Mårtensson A, Alfvén T, Gantelius J, Andersson-Svahn H
Talanta 183 (-) 192-200 [2018-06-01; online 2018-02-16]
Paper-based biosensors offer a promising technology to be used at the point of care, enabled by good performance, convenience and low-cost. In this article, we describe a colorimetric vertical-flow DNA microarray (DNA-VFM) that takes advantage of the screening capability of DNA microarrays in a paper format together with isothermal amplification by means of Recombinase Polymerase Amplification (RPA). Different assay parameters such as hybridization buffer, flow rate, printing buffer and capture probe concentration were optimized. A limit of detection (LOD) of 4.4 nM was achieved as determined by tabletop scanning. The DNA-VFM was applied as a proof of concept for detection of Neisseria meningitidis, a primary cause of bacterial meningitis. The LOD was determined to be between 38 and 2.1 × 10
PubMed 29567164
DOI 10.1016/j.talanta.2018.02.070
Crossref 10.1016/j.talanta.2018.02.070
pii: S0039-9140(18)30178-4