Engineered Hydrogels for 3D Cell Culture and Bioprinting of Human Induced Pluripotent Stem Cell-Derived Neuroepithelial Stem Cells.
JSON
Methods in molecular biology (Clifton, N.J.) 2924 (-) 223-233 [2025-05-01; online 2025-05-01]
This protocol outlines the synthesis and use of engineered hyaluronan-based hydrogels for 3D cell culture and bioprinting of human induced pluripotent stem cell (hiPSC)-derived neuroepithelial stem cells (lt-NES). Key steps include hydrogel formation using bioorthogonal chemistries, cell encapsulation, and 3D bioprinting with a Cellink BioX printer, enabling the creation of complex tissue models. The protocol ensures high cell viability and supports differentiation, essential for neuroscience research and drug development.
PubMed 40307646
DOI 10.1007/978-1-0716-4530-7_16
Crossref 10.1007/978-1-0716-4530-7_16