{"entity": "publication", "iuid": "96394b2621824250ac9457178565a99d", "timestamp": "2026-10-01T11:59:01.933Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/96394b2621824250ac9457178565a99d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/96394b2621824250ac9457178565a99d"}}, "title": "Engineered Hydrogels for 3D Cell Culture and Bioprinting of Human Induced Pluripotent Stem Cell-Derived Neuroepithelial Stem Cells.", "authors": [{"family": "Aili", "given": "Daniel", "initials": "D", "orcid": "0000-0002-7001-9415", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3624d34bc7734e329c0d4b465b5ac567.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2025-05-01", "journal": {"title": "Methods in molecular biology (Clifton, N.J.)", "issn": "1940-6029", "volume": "2924", "pages": "223-233", "issn-l": "1064-3745"}, "abstract": "This protocol outlines the synthesis and use of engineered hyaluronan-based hydrogels for 3D cell culture and bioprinting of human induced pluripotent stem cell (hiPSC)-derived neuroepithelial stem cells (lt-NES). Key steps include hydrogel formation using bioorthogonal chemistries, cell encapsulation, and 3D bioprinting with a Cellink BioX printer, enabling the creation of complex tissue models. The protocol ensures high cell viability and supports differentiation, essential for neuroscience research and drug development.", "doi": "10.1007/978-1-0716-4530-7_16", "pmid": "40307646", "labels": [], "xrefs": [], "notes": [], "created": "2026-10-01T08:30:28.801Z", "modified": "2026-10-01T08:30:28.860Z"}