Recognizing uncertainty increases robustness and reproducibility of mass spectrometry-based protein inferences.
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Serang O, Moruz L, Hoopmann MR, Käll L
J. Proteome Res. 11 (12) 5586-5591 [2012-12-07; online 2012-11-19]
Parsimony and protein grouping are widely employed to enforce economy in the number of identified proteins, with the goal of increasing the quality and reliability of protein identifications; however, in a counterintuitive manner, parsimony and protein grouping may actually decrease the reproducibility and interpretability of protein identifications. We present a simple illustration demonstrating ways in which parsimony and protein grouping may lower the reproducibility or interpretability of results. We then provide an example of a data set where a probabilistic method increases the reproducibility and interpretability of identifications made on replicate analyses of Human Du145 prostate cancer cell lines.
PubMed 23148905
DOI 10.1021/pr300426s
Crossref 10.1021/pr300426s