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Garrido-Godino AI, Gupta I, Pelechano V, Navarro F
Int J Mol Sci 25 (1) - [2023-12-29; online 2023-12-29]
RNA pol II assembly occurs in the cytoplasm before translocation of the enzyme to the nucleus. Affecting this assembly influences mRNA transcription in the nucleus and mRNA decay in the cytoplasm. However, very little is known about the consequences on ncRNA synthesis. In this work, we show that impairment of RNA pol II assembly leads to a decrease in cryptic non-coding RNAs (preferentially CUTs and SUTs). This alteration is partially restored upon overcoming the assembly defect. Notably, this drop in ncRNAs is only partially dependent on the nuclear exosome, which suggests a major specific effect of enzyme assembly. Our data also point out a defect in transcription termination, which leads us to propose that CTD phosphatase Rtr1 could be involved in this process.
PubMed 38203678
DOI 10.3390/ijms25010507
Crossref 10.3390/ijms25010507
pmc: PMC10778713
pii: ijms25010507