Systematic approach for dissecting the molecular mechanisms of transcriptional regulation in bacteria.

Belliveau NM, Barnes SL, Ireland WT, Jones DL, Sweredoski MJ, Moradian A, Hess S, Kinney JB, Phillips R

Proc. Natl. Acad. Sci. U.S.A. 115 (21) E4796-E4805 [2018-05-22; online 2018-05-04]

Gene regulation is one of the most ubiquitous processes in biology. However, while the catalog of bacterial genomes continues to expand rapidly, we remain ignorant about how almost all of the genes in these genomes are regulated. At present, characterizing the molecular mechanisms by which individual regulatory sequences operate requires focused efforts using low-throughput methods. Here, we take a first step toward multipromoter dissection and show how a combination of massively parallel reporter assays, mass spectrometry, and information-theoretic modeling can be used to dissect multiple bacterial promoters in a systematic way. We show this approach on both well-studied and previously uncharacterized promoters in the enteric bacterium

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PubMed 29728462

DOI 10.1073/pnas.1722055115

Crossref 10.1073/pnas.1722055115

1722055115