Far-Field Autofluorescence Nanoscopy

Bierwagen J, Testa I, FoĢˆlling J, Wenzel D, Jakobs S, Eggeling C, Hell SW

Nano Lett. 10 (10) 4249-4252 [2010-10-13; online 2010-10-13]

We demonstrate far-field optical imaging at the nanoscale with unlabeled samples. Subdiffraction resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning. Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on-off cycle to yield an image, a condition fulfilled by various biomolecules. The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts.

Ilaria Testa

SciLifeLab Fellow

PubMed 20831171

DOI 10.1021/nl1027638

Crossref 10.1021/nl1027638

Publications 9.5.0