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Bierwagen J, Testa I, FoĢlling J, Wenzel D, Jakobs S, Eggeling C, Hell SW
Nano Lett. 10 (10) 4249-4252 [2010-10-13; online 2010-10-13]
We demonstrate far-field optical imaging at the nanoscale with unlabeled samples. Subdiffraction resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning. Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on-off cycle to yield an image, a condition fulfilled by various biomolecules. The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts.
PubMed 20831171
DOI 10.1021/nl1027638
Crossref 10.1021/nl1027638