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Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto T
Nat. Methods 13 (10) 823-827 [2016-10-00; online 2016-09-05]
We convened an ad hoc International Working Group for Antibody Validation in order to formulate the best approaches for validating antibodies used in common research applications and to provide guidelines that ensure antibody reproducibility. We recommend five conceptual ‘pillars’ for antibody validation to be used in an application-specific manner.
PubMed 27595404
DOI 10.1038/nmeth.3995
Crossref 10.1038/nmeth.3995
mid: NIHMS1910904
pmc: PMC10335836
pii: nmeth.3995