Robust, universal biomarker assay to detect senescent cells in biological specimens.

Evangelou K, Lougiakis N, Rizou SV, Kotsinas A, Kletsas D, Muñoz-Espín D, Kastrinakis NG, Pouli N, Marakos P, Townsend P, Serrano M, Bartek J, Gorgoulis VG

Aging Cell 16 (1) 192-197 [2017-02-00; online 2016-11-17]

Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.

Affiliated researcher

PubMed 28165661

DOI 10.1111/acel.12545

Crossref 10.1111/acel.12545

pmc: PMC5242262

Publications 9.5.0