Applying 3D cultures and high-throughput technologies to study host-pathogen interactions.

De Martinis ECP, Alves VF, Pereira MG, Andrade LN, Abichabki N, Abramova A, Dannborg M, Bengtsson-Palme J

Front Immunol 16 (-) 1488699 [2025-02-20; online 2025-02-20]

Recent advances in cell culturing and DNA sequencing have dramatically altered the field of human microbiome research. Three-dimensional (3D) cell culture is an important tool in cell biology, in cancer research, and for studying host-microbe interactions, as it mimics the in vivo characteristics of the host environment in an in vitro system, providing reliable and reproducible models. This work provides an overview of the main 3D culture techniques applied to study interactions between host cells and pathogenic microorganisms, how these systems can be integrated with high-throughput molecular methods, and how multi-species model systems may pave the way forward to pinpoint interactions among host, beneficial microbes and pathogens.

DDLS Fellow

Johan Bengtsson-Palme

PubMed 40051624

DOI 10.3389/fimmu.2025.1488699

Crossref 10.3389/fimmu.2025.1488699

pmc: PMC11882522


Publications 9.5.1