Monitoring Conformational Dynamics with Single-Molecule Fluorescence Energy Transfer: Applications in Nucleosome Remodeling
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Deindl S, Zhuang X
- 513 (-) 59-86 [2012-00-00; online 2012-00-00]
Due to its ability to track distance changes within individual molecules or molecular complexes on the nanometer scale and in real time, single-molecule fluorescence resonance energy transfer (single-molecule FRET) is a powerful tool to tackle a wide range of important biological questions. Using our recently developed single-molecule FRET assay to monitor nucleosome translocation as an illustrative example, we describe here in detail how to set up, carry out, and analyze single-molecule FRET experiments that provide time-dependent information on biomolecular processes.
PubMed 22929765
DOI 10.1016/b978-0-12-391938-0.00003-3
Crossref 10.1016/b978-0-12-391938-0.00003-3