Monitoring Conformational Dynamics with Single-Molecule Fluorescence Energy Transfer: Applications in Nucleosome Remodeling

Deindl S, Zhuang X

- 513 (-) 59-86 [2012-00-00; online 2012-00-00]

Due to its ability to track distance changes within individual molecules or molecular complexes on the nanometer scale and in real time, single-molecule fluorescence resonance energy transfer (single-molecule FRET) is a powerful tool to tackle a wide range of important biological questions. Using our recently developed single-molecule FRET assay to monitor nucleosome translocation as an illustrative example, we describe here in detail how to set up, carry out, and analyze single-molecule FRET experiments that provide time-dependent information on biomolecular processes.

Sebastian Deindl

PubMed 22929765

DOI 10.1016/b978-0-12-391938-0.00003-3

Crossref 10.1016/b978-0-12-391938-0.00003-3

Publications 7.1.2