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Fornell A, Ohlin M, Garofalo F, Nilsson J, Tenje M
Biomicrofluidics 11 (3) 031101 [2017-05-00; online 2017-05-26]
To transfer cell- and bead-assays into droplet-based platforms typically requires the use of complex microfluidic circuits, which calls for methods to switch the direction of the encapsulated particles. We present a microfluidic chip where the combination of acoustic manipulation at two different harmonics and a trident-shaped droplet-splitter enables direction-switching of microbeads and yeast cells in droplet microfluidic circuits. At the first harmonic, the encapsulated particles exit the splitter in the center daughter droplets, while at the second harmonic, the particles exit in the side daughter droplets. This method holds promises for droplet-based assays where particle-positioning needs to be selectively controlled.
PubMed 28580044
DOI 10.1063/1.4984131
Crossref 10.1063/1.4984131
pii: 1.4984131
pii: 016703BMF
pmc: PMC5446280