Multifocus structured illumination microscopy for fast volumetric super-resolution imaging.

Abrahamsson S, Blom H, Agostinho A, Jans DC, Jost A, Müller M, Nilsson L, Bernhem K, Lambert TJ, Heintzmann R, Brismar H

Biomed Opt Express 8 (9) 4135-4140 [2017-09-01; online 2017-08-17]

We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in super-resolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens.

Affiliated researcher

PubMed 28966852

DOI 10.1364/BOE.8.004135

Crossref 10.1364/BOE.8.004135

pii: 294866
pmc: PMC5611928
figshare: 10.6084/m9.figshare.4928747

Publications 9.5.0