In situ sequencing for RNA analysis in preserved tissue and cells.

Ke R, Mignardi M, Pacureanu A, Svedlund J, Botling J, Wählby C, Nilsson M

Nat. Methods 10 (9) 857-860 [2013-09-00; online 2013-07-14]

Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. We have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. We demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.

Affiliated researcher

PubMed 23852452

DOI 10.1038/nmeth.2563

Crossref 10.1038/nmeth.2563

pii: nmeth.2563

Publications 9.5.0