Dual channel RESOLFT nanoscopy by using fluorescent state kinetics.

Testa I, D'Este E, Urban NT, Balzarotti F, Hell SW

Nano Lett. 15 (1) 103-106 [2015-01-14; online 2014-12-02]

We show that RESOLFT fluorescence nanoscopy, a low light level scanning superresolution technique employing reversibly switchable fluorescent proteins (rsFPs), is capable of dual-channel live-cell imaging that is virtually free of chromatic errors and temporal offsets. This is accomplished using rsEGFP and Dronpa, two rsFPs having similar spectra but different kinetics of switching and fluorescence emission. Our approach is demonstrated by imaging protein distributions and dynamics in living neurons and neuronal tissues.

Ilaria Testa

SciLifeLab Fellow

PubMed 25423166

DOI 10.1021/nl503058k

Crossref 10.1021/nl503058k

Publications 9.5.0